Background: Ayurved Siriraj Wattana recipe (AVS073), has been prescribed as tonic, to increase appetite, and for\npain relief. It also exhibits antioxidant, anti-inflammatory, immunomodulating and anti-cancer activities.\nHowever, the immunomodulatory effects on antigen-presenting cells and effector T cells remained elusive.\nWe thus aimed to study the effects of AVS073 on differentiation, maturation, functions and proportions\nof CIK cells and monocyte-derived DCs.\nMethods: CIK cells and monocyte-derived DCs were treated with AVS073, followed by the assessment of\nT-helper (Th) phenotypes using real-time RT-PCR and flow cytometry.\nResults: AVS073 promoted Th1 phenotype in CD3+CD56+ subset of CIK cells through increasing STAT4,\nT-bet, and interferon-�³. AVS073 inhibited Th2 phenotype through decreasing STAT6. AVS073 inhibited Treg\nphenotype through decreasing STAT5A, STAT5B and IDO. AVS073 promoted Th17 phenotype through\nincreasing STAT3, RORC and IL-17. AVS073 treatment of mDCs resulted in increasing Th1-prone cytokine\n(IL-12) and Th17-prone cytokines (IL-6 and IL-23).\nConclusions: AVS073 upregulated Th1 and Th17, but downregulated Th2 and Treg phenotypes within CD3\n+CD56+ cells. The treatment of mDCs drove Th1 and Th17-polarizations.
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